Cell Based Assays
Promega FES Kinase Enzyme System, 10microg
Recombinant full-length human FES was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FES is a proto-oncogene that encodes a protein-tyrosine kinase distinct from c-Src, c-Abl and other nonreceptor tyrosine kinases.
Promega ADP-Glo™ Kinase Assay + PKA Kinase Enzyme System
Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.
Promega MST1 Kinase Enzyme System, 1mg
Recombinant full-length human MST1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20).
Thermo Scientific™ Jump-In™ GripTite™ HEK 293 Kit
The Jump-In™ GripTite™ HEK293 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ GripTite™ HEK293 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.
Promega ITK Kinase Enzyme System, 1mg
Recombinant human ITK (amino acids 352-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ITK is a member of the TEC family of nonreceptor tyrosine kinases.
Promega ADP-Glo™ Kinase Assay + PIM1 Kinase Enzyme System
Recombinant full-length human PIM1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM1 is a proto-oncogene that belongs to a family of serine/threonine protein kinases that are highly conserved in multicellular organisms.
Promega Kinase-Glo™ Luminescent Kinase Assay
The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Promega ADP-Glo™ Kinase Assay + PASK Kinase Enzyme System
Recombinant human PASK (981-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK or PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress.
Promega ADP-Glo™ Kinase Assay + LCK Kinase Enzyme System
Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LCK (p56lck) is a member of the src family of nonreceptor tyrosine kinases.
Promega ZAK Kinase Enzyme System, 10microg
Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.
Promega ADP-Glo™ Kinase Assay + AKT3 Kinase Enzyme System
Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT3 or Protein Kinase B gamma (PKBgamma) is a serine/threonine kinase that is a member of the AKT family.
Promega ADP-Glo™ Kinase Assay + ABL1 (E255K) Kinase Enzyme System
Recombinant human ABL1 (E255K) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega AKT2 Kinase Enzyme System, 10microg
Recombinant full-length human AKT2 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. AKT2 or Protein Kinase B beta (PKB beta) is a serine/threonine kinase that is a member of the AKT family.
Promega p38 gamma Kinase Enzyme System, 1mg
Recombinant full-length human p38 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 gamma is a member of the p38 MAPK family, which is activated in response to stress.
Promega RET (Y791F) Kinase Enzyme System, 1mg
Recombinant human RET (Y791F) (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.